Get 3D image data at scale
The ZOMP Parallax is the world’s first 3D imaging flow cytometer. Each cell’s full 3D spatial information is recorded with imaging quality unmatched by any other cytometer on the market. This unique technology allows biologists to capture the spatial information of entire cells at population scale, enabling new studies and ensuring more confidence in results.
An example 3D image:
High confidence measurements:
- Colocalisation
- Translocation
- Internalisation
- Spot counting
- Morphology
- Cell-cell interaction
- Subcellular interaction (e.g. protein-protein)
- All standard flow cytometry measurements
Instrument Specification
| Imaging Modes | Subcellular | Full-cell |
|---|---|---|
| Application: | feature localisation, spot counting, target–organelle colocalisation, cell cycle | morphology, target internalisation, cell-cell interaction |
| Resolution: | 0.8 µm | 1.2 µm |
| Pixel size: | 0.4 µm | 0.6 µm |
| Depth-of-field (z): | 12 µm | 25 µm |
| Field-of-view (x,y): | 55 µm | 55 µm |
Optics
Excitation lasers
405 nm – 200 mW
445 nm – 75 mW (SSC only)
488 nm – 200 mW
561 nm – 200 mW
638 nm – 160 mW
Detection channels
Colour channels: 445/40–525/50–600/40–680/40
Limitation: up to 4 fluorescent labels + SSC and Bright Field imaging
Example panel: DAPI – FITC or PE – APC
Label free detection
Dedicated 3D side-scatter imaging channel (3D-SSC)
Transmission brightfield imaging available in any colour channel
Fluidics
Up to 1000 events/second, if every frame has an object
Exchangeable microfluidic chip for hydrodynamic focusing
1.5 mL tube sample input (min. required volume 100 µL)
50 mL sheath, cleaning and waste reservoirs
Automated sample loading and cleaning procedures
Integrated pressure pumps